One-Step Quantitative RT-PCR Assay with Armored RNA Controls

One-Step Quantitative RT-PCR Assay with Armored RNA Controls for Detection of SARS-CoV-2

COVID-19 has develop to be pandemic since March, 11, 2020. Thus, development and integration in clinics of fast and delicate diagnostic devices is essential. The aim of the analysis was a development and evaluation of a one-step RT-qPCR assay (COVID-19 Amp) for SARS-CoV-2 detection with an armored optimistic administration and inside controls constructed from synthetic MS2-phage-based RNA particles.

The COVID-19 Amp assay prohibit of detection was 103 copies/ml, the analytical specificity was 100%. 109 natural samples have been examined using COVID-19 Amp and WHO-based assay.

Discordance in 9 samples was observed (unfavourable by the WHO-based assay) and discordant samples have been retested as optimistic based mostly on the outcomes obtained from the Vector-PCRrv-2019-nCoV-RG assay.

The developed COVID-19 Amp assay has extreme sensitivity and specificity, incorporates virus particles-based controls, provides the direct definition of SARS-CoV-2 RdRp gene partial sequence, and is acceptable for any hospital and laboratory equipped for RT-qPCR. This textual content is protected by copyright. All rights reserved.

Results of graphene oxide on PCR amplification for microbial group survey

Background: Graphene oxide (GO) has been advised as an environment friendly assistant additive to eradicate non-specific amplification of the polymerase chain response (PCR). Though many research have centered on exploring its molecular mechanism, the apply of GO on the quantitation of microbial group has not been carried out but. On this examine, GO was added in PCR system to discover the adjustments on eradicating typical amplification errors, reminiscent of chimera and mismatches on two sorts of mock communities (an evenly blended and a staggered mock communities) and environmental samples

Outcomes: Excessive-throughput sequencing of bacterial and fungal communities, based mostly on 16S rRNA genes and inside transcribed spacers (ITS) respectively, confirmed that GO may considerably enhance giant segmental error (chimeric sequence) in PCR process whereas had no particular impact on level error (mismatched sequence). Moreover, GO decreased the α-diversity of group, and altered the composition of fungal group extra clearly than bacterial group.

Conclusions: Our examine offers the primary quantitative knowledge on microbial group degree to show the unfavourable impact of GO, and likewise signifies that there could also be a extra complicated interplay between GO and complete DNA fragments in PCR course of.

q-es
q-es

Frozen Tissue Section - hypertension: Vein

T1236020Hd-2 5 slides
EUR 465
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from hypertension: Vein

P1236020Hd-2 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human ; Umbilical Vein Endothelial Cells

02-701 100ng
EUR 401
Description: The Human ; Umbilical Vein Endothelial Cells is available in Europe and for worldwide shipping via Gentaur.

Human Umbilical Vein Endothelial Cells

HEC01 500,000+ Cells frozen
EUR 440

Human Saphenous Vein Endothelial Cells

HEC18 500,000+ Cells
EUR 1080

Rat Heart PrimaCell5: Normal Saphenous Vein Endothelial Cells

2-82550 1 Kit Ask for price

Rat Intestine PrimaCell3: Normal Itestinal Vein Endothelial Cells

2-82554 1 Kit Ask for price

Rat Lung PrimaCell5: Normal Pulmonary Vein Endothelial Cells

2-82568 1 Kit Ask for price

cDNA from Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Kidney

C1434142 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Placenta

C1434200 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Rat Lung PrimaCell6: Normal Pulmonary Vein Smooth Muscle Cells

2-82569 1 Kit Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Skeletal Muscle

C1434171 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Rat Heart PrimaCell5: Normal Saphenous Vein Endothelial Cells Growth Medium

9-25050 5 x 100 ml Ask for price

Rat Intestine PrimaCell3: Normal Itestinal Vein Endothelial Cells Growth Medium

9-25054 5 x 100 ml Ask for price

Rat Lung PrimaCell5: Normal Pulmonary Vein Endothelial Cells Growth Medium

9-25068 5 x 100 ml Ask for price

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells

2-82587 1 Kit Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415

Immortalized Human Umbilical Vein Endothelial Cells - SV40

T0014 1x106 cells / 1.0 ml Ask for price

GFP Expressing Human Umbilical Vein Endothelial Cells

GF01 500,000+ Cells frozen
EUR 1040

Rat Lung PrimaCell6: Normal Pulmonary Vein Smooth Muscle Cells Growth Medium

9-25069 5 x 100 ml Ask for price

Rat Heart Tissue Preparation Buffer 5: Normal Saphenous Vein Endothelial Cells

9-80257 1 x 100 ml Ask for price

Rat Intestine Tissue Preparation Buffer 3: Normal Itestinal Vein Endothelial Cells

9-80261 1 x 100 ml Ask for price

Rat Lung Tissue Preparation Buffer 5: Normal Pulmonary Vein Endothelial Cells

9-80275 1 x 100 ml Ask for price

Mouse Heart PrimaCell5: Normal Saphenous Vein Endothelial Cells

2-82049 1 Kit Ask for price

Mouse Intestine PrimaCell5: Normal Itestinal Vein Endothelial Cells

2-82055 1 Kit Ask for price

Mouse Lung PrimaCell6: Normal Pulmonary Vein Endothelial Cells

2-82069 1 Kit Ask for price

Human Heart PrimaCell4: Normal Saphenous Vein Endothelial Cells

2-96069 1 Kit Ask for price

Human Intestine PrimaCell3: Normal Itestinal Vein Endothelial Cells

2-96073 1 Kit Ask for price

Human Lung PrimaCell5: Normal Pulmonary Vein Endothelial Cells

2-96085 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, Biotinylated

AP-316-B 1 mg Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, unlabeled

AP-316-U 5 mg Ask for price

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 77.76

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 134

amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
EUR 192

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 445

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 415

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 415

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 415

Human Adult cDNA Tissue: Testis

HA-260 10 rxn
EUR 415

Human Tumor Tissue: Breast cDNA

HT05-090 10 rxn
EUR 415

Rat Umbilical Cord PrimaCell: Normal Umbilical Vein Smooth Muscle Cells Growth Medium

9-25087 5 x 100 ml Ask for price

Rat Lung Tissue Preparation Buffer 6: Normal Pulmonary Vein Smooth Muscle Cells

9-80276 1 x 100 ml Ask for price

Rat Umbilical Cord Tissue Preparation Buffer: Normal Umbilical Vein Smooth Muscle Cells

9-80294 1 x 100 ml Ask for price

Frozen Tissue Section - Human Adult Normal: Blood Vessel: Vein

T1234020 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Blood Vessel: Vein

T2234020 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Genomic DNA from hypertension: Vein, from a single donor

D1236020Hd-2 50 ug
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Mouse Lung PrimaCell7: Normal Pulmonary Vein Smooth Muscle Cells

2-82070 1 Kit Ask for price

Human Lung PrimaCell6: Normal Pulmonary Vein Smooth Muscle Cells

2-96086 1 Kit Ask for price

Human Umbilical Cord PrimaCell4: Normal Umbilical Vein Endothelial Cells

2-96109 1 Kit Ask for price

human umbilical vein endothelial cells, Peptide Aptamer, FITC labelled

AP-316-F 1 mg Ask for price

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 786

cDNA from Human Tumor Tissue: Adrenal

C1235004-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bladder

C1235010-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bone

C1235023-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Brain

C1235035-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Breast

C1235086 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Colon

C1235090 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Liver

C1235149 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lung

C1235152 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lymphoma

C1235161-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Mesenchymoma

C1235164-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Nose

C1235178-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Ovary

C1235183-10 10 reactions
EUR 494
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Pancreas

C1235188-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Parathyroid

C1235189-10 10 reactions
EUR 494
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Prostate

C1235201-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Rectum

C1235206-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Skin

C1235218-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Melanoma

C1235218A-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Stomach

C1235248 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Uterus

C1235274 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Brain

C1236035Dia 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Multiple Sclerosis Disease: Brain

C1236035Msc 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Amygdala

C1236036Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Cerebellum

C1236039Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Hippocampus

C1236052Alz 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain: Hippocampus

C1236052Par 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Pons

C1236071Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Thalamus

C1236079Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Routine blood evaluation drastically reduces the false-negative price of RT-PCR testing for Covid-19

Background: The COVID-19 outbreak is now a pandemic illness reaching as a lot as 210 international locations worldwide with greater than 2.5 million contaminated folks and almost 200.000 deaths. Amplification of viral RNA by RT-PCR represents the gold commonplace for affirmation of an infection, but it confirmed false-negative charges as giant as 15-20% which could jeopardize the impact of the restrictive measures taken by governments. We beforehand confirmed that a number of hematological parameters had been considerably totally different between COVID-19 constructive and unfavourable sufferers. Amongst them aspartate aminotransferase and lactate dehydrogenase had predictive values as giant as 90%. Thus a mix of RT-PCR and blood assessments may cut back the false-negative price of the genetic check.

Strategies: We retrospectively analyzed 24 sufferers displaying a number of and inconsistent RT-PCR, check throughout their first hospitalization interval, and in contrast the genetic assessments outcomes with their AST and LDH ranges.

Outcomes: We confirmed that when contemplating the hematological parameters, the RT-PCR false-negative charges had been decreased by virtually 4-fold.

Conclusions: The examine represents a preliminary work aiming on the improvement of methods that, by combining RT-PCR assessments with routine blood assessments, will decrease and even abolish the speed of RT-PCR false-negative outcomes and thus will establish, with excessive accuracy, sufferers contaminated by COVID-19.

Multiplex detection of ctDNA mutations in plasma of colorectal most cancers sufferers by PCR/SERS assay
 

Molecular diagnostic testing of KRAS and BRAF mutations has develop into important within the administration of colorectal most cancers (CRC) sufferers. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), however sluggish evaluation for DNA sequencing strategies has restricted fast diagnostics.

Different strategies reminiscent of quantitative PCR and extra lately, droplet digital PCR (ddPCR), have limitations in multiplexed capability and the necessity for costly specialised tools. Therefore, a strong, fast and facile technique is required for detecting a number of ctDNA mutations to enhance the administration of CRC sufferers. To handle this important downside, herein, we suggest a brand new software of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a quick and non-invasive method to diagnose and stratify sufferers for efficient remedy.

Strategies: To discriminate ctDNA mutations from wild-type cfDNA, allele-specific primers had been designed for the amplification of three clinically essential DNA level mutations in CRC together with KRAS G12V, KRAS G13D and BRAF V600E. Floor-enhanced Raman scattering (SERS) nanotags had been labelled with a brief and particular sequence of oligonucleotide, which may hybridize with the corresponding PCR amplicons. The PCR/SERS assay was carried out by firstly amplifying the a number of mutations, adopted by binding with multicolor SERS nanotags particular to every mutation, and subsequent enrichment with magnetic beads. The mutation standing was evaluated utilizing a transportable Raman spectrometer the place the fingerprint spectral peaks of the corresponding SERS nanotags point out the presence of the mutant targets. The tactic was then utilized to detect ctDNA from CRC sufferers beneath a blinded check, the outcomes had been additional validated by ddPCR.

Outcomes: The PCR/SERS technique confirmed excessive specificity and sensitivity for genotyping CRC cell strains and plasma ctDNA, the place as few as 0.1% mutant alleles may very well be detected from a background of plentiful wild-type cfDNA. The blinded check utilizing 9 samples from superior CRC sufferers by PCR/SERS assay was validated with ddPCR and confirmed good consistency with pathology testing outcomes.

Conclusions: With ddPCR-like sensitivity but on the comfort of ordinary PCR, the proposed assay reveals nice potential in delicate detection of a number of ctDNA mutations for scientific decision-making.

Add a Comment

Your email address will not be published.