A Comparative Research of Some Procedures for Isolation of Fruit DNA of Enough High quality

A Comparative Analysis of Some Procedures for Isolation of Fruit DNA of Sufficient Top quality for PCR-Based Assays

  • Meals fraud has been and nonetheless is a matter throughout the meals commerce. It is detectable by plenty of approaches, harking back to extreme effectivity liquid chromatography (HPLC), chemometric assays, or DNA-based methods, each with its private drawbacks. This work addresses one major drawback of DNA-based methods, significantly their sensitivity to inhibitors contained significantly matrices from which DNA is isolated.
  • We examined 5 enterprise kits and one in-house approach characterised by alternative routes of sample homogenization and DNA seize and purification.
  • Using these methods, DNA was isolated from 10 utterly completely different fruit species typically utilized in plant-based foodstuffs. The usual of the DNA was evaluated by UV-VIS spectrophotometry. Two sorts of qPCR assays have been used for DNA top quality testing: (i) Approach explicit for plant ITS2 space, (ii) methods explicit for explicit individual fruit species.
  • Based totally on the outcomes of real-time PCR assays, we now have been able to find two column-based kits and one magnetic carrier-based gear, which always equipped fruit DNA isolates of sufficient top quality for PCR-based assays useful for routine analysis and identification of explicit individual fruit species in meals merchandise.

Triplex real-time PCR assay for the authentication of camel-derived dairy and meat merchandise 

Authentication of dairy and meat merchandise is necessary to guarantee truthful competitors, client profit, and meals security. The big distinction in value between camel and cow milk could also be an incentive to adulterate camel dairy merchandise with cow-derived foodstuffs. Nevertheless, no research thus far have used triplex real-time PCR with an endogenous management to establish camel and cow origins in dairy and meat merchandise.

On this examine, we developed a triplex real-time PCR assay primarily based on amplification of mitochondrial 12S ribosomal DNA for the authentication of camel-derived dairy and meat merchandise. This technique was utilized to establish camel and cow DNA in milk, yogurt, cheese, milk powder, milk beverage, meat merchandise, and mixtures with milk and meat. Concentrations as little as 1 to five% and 0.1% camel milk and meat, respectively, had been detected within the mixtures, and 1 to five% and 0.1% cow milk and meat, respectively, had been recognized by way of this strategy.

The boundaries of detection had been 0.005 to 0.0025 ng, 0.05 to 0.001 ng, 0.001 to 0.0005 ng, and 0.00025 to 0.0001 ng of DNA in camel milk, camel yogurt, business camel milk beverage, and camel meat, and from 0.0025 to 0.001 ng, 0.5 to 0.001 ng, 1 to 0.05 ng, 0.01 ng, 0.001 ng, 0.0005 to 0.00025 ng, 0.0005 to 0.00025 ng, and 0.005 ng of DNA from cow milk, yogurt, cheese, acidic whey, milk powder, beef, beef jerky, and beef sausage, respectively. Totally different dairy and meat samples of camel and cow origins had a spread of authentication limits and limits of detection. The designed triplex real-time PCR assay was proven to be a selected, delicate, and environment friendly approach for the identification of camel and cow DNA in foodstuffs.

q-es
q-es

cDNA from Human Adult Normal Tissue: Uterus: Cervix

C1234275 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus: Corpus

C1234276-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus: Fundus

C1234278-10 10 reactions
EUR 231
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Cervix

C1534275 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix

C1534275-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-48Tests 48 Tests
EUR 436

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-96Tests 96 Tests
EUR 601

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-48Tests 48 Tests
EUR 533

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-96Tests 96 Tests
EUR 740

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-48Tests 48 Tests
EUR 557

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-96Tests 96 Tests
EUR 775

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-48T 48T
EUR 441
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-96T 96T
EUR 570
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-48T 48T
EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-96T 96T
EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-48T 48T
EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-96T 96T
EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-48Tests 48 Tests
EUR 455

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-96Tests 96 Tests
EUR 629

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-48Tests 48 Tests
EUR 557

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-96Tests 96 Tests
EUR 774

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-48Tests 48 Tests
EUR 583

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-96Tests 96 Tests
EUR 811

Uterus Lysate

1317 0.1 mg
EUR 191
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 527
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 516.5
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tumor Lysate

1384 0.1 mg
EUR 254
Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Bovine UTERUS 5 EA*

57153-2 5 EA
EUR 204.2

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 773

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 201.5

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 180.5

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 201.5

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 180.5

Uterus Tissue Slide (Adenocarcinoma)

11-404-10um 10 um
EUR 201.5

Uterus Tissue Slide (Adenocarcinoma)

11-404-4um 4 um
EUR 180.5

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 201.5

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 180.5

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 201.5

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 180.5

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 626.75
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Corpus Membrane Lysate

XBL-11037 0.1 mg
EUR 516.5
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate

XBL-11040 0.1 mg
EUR 516.5
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

CAT/ Rat CAT ELISA Kit

ELA-E0242r 96 Tests
EUR 886

Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

CAT Antibody

1-CSB-PA004563GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CAT Antibody

1-CSB-PA004980
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

CAT Antibody

1-CSB-PA070043
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000

CAT Antibody

1-CSB-PA942303
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

CAT Antibody

1-CSB-PA599838
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200

CAT Antibody

36312-100ul 100ul
EUR 252

CAT siRNA

20-abx910366
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx910367
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx900807
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

CAT antibody

20C-CR1111RP 500 ul
EUR 262
Description: Rabbit polyclonal CAT antibody

CAT antibody

10R-3584 100 ul
EUR 726
Description: Mouse monoclonal CAT antibody

CAT antibody

10R-3585 100 ul
EUR 691
Description: Mouse monoclonal CAT antibody

CAT antibody

70R-16187 50 ul
EUR 435
Description: Rabbit polyclonal CAT antibody

Cat IgM

31C-CH0213 1 mg
EUR 392
Description: Purified Cat IgM

pOTB7-CAT

PVT18217 2 ug
EUR 231

pCas9- CAT

PVT11001 2 ug
EUR 301

Rabbit UTERUS Y 25 EA*

41253-2 25 EA
EUR 318.39

Total RNA from Lupus: Uterus

R1236274Lup-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-10um 10 um
EUR 201.5

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-4um 4 um
EUR 180.5

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 328
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Catalase (10,000-15,000 U/mg), Bovine Liver

CAT-01 25 mU
EUR 529

Catalase (2000-4000 U/mg), Aspergillus Niger

CAT-02 25 mU
EUR 651

Catalase (4000-6000 U/mg), Bovine Liver

CAT-03 5 mU
EUR 347

Catalase (>40,000 u/mg), Bovine Liver

CAT-04 1 mg
EUR 286

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-10um 10 um
EUR 201.5

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-4um 4 um
EUR 180.5

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-10um 10 um
EUR 201.5

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-4um 4 um
EUR 180.5

Recombinant Catalase (CAT)

4-RPC418Hu01
  • EUR 279.20
  • EUR 178.00
  • EUR 772.00
  • EUR 324.00
  • EUR 548.00
  • EUR 250.00
  • EUR 1780.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Catalase expressed in: E.coli

Recombinant Catalase (CAT)

4-RPC418Hu02
  • EUR 279.20
  • EUR 178.00
  • EUR 772.00
  • EUR 324.00
  • EUR 548.00
  • EUR 250.00
  • EUR 1780.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Catalase expressed in: E.coli

Recombinant Catalase (CAT)

4-RPC418Hu03
  • EUR 279.20
  • EUR 178.00
  • EUR 772.00
  • EUR 324.00
  • EUR 548.00
  • EUR 250.00
  • EUR 1780.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Catalase expressed in: E.coli

Recombinant Catalase (CAT)

4-RPC418Hu04
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Catalase expressed in: E.coli

Recombinant Catalase (CAT)

4-RPC418Mu01
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Catalase expressed in: E.coli

Recombinant Catalase (CAT)

4-RPC418Mu02
  • EUR 368.80
  • EUR 202.00
  • EUR 1108.00
  • EUR 436.00
  • EUR 772.00
  • EUR 310.00
  • EUR 2620.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Catalase expressed in: E.coli

CAT cloning plasmid

CSB-CL004563HU-10ug 10ug
EUR 376
Description: A cloning plasmid for the CAT gene.

Anti-CAT antibody

STJ27228 100 µl
EUR 277
Description: This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Anti-CAT antibody

STJ119991 100 µl
EUR 413
Description: This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Anti-CAT antibody

STJ113361 100 µl
EUR 277
Description: This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Anti-CAT antibody

STJ113364 100 µl
EUR 277
Description: This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene.

Catalase (CAT) Antibody

20-abx125619
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx125620
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx111432
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx323535
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx004037
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

Catalase (CAT) Antibody

20-abx100146
  • EUR 356.00
  • EUR 133.00
  • EUR 996.00
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100147
  • EUR 356.00
  • EUR 133.00
  • EUR 996.00
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100148
  • EUR 356.00
  • EUR 133.00
  • EUR 996.00
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100149
  • EUR 356.00
  • EUR 133.00
  • EUR 996.00
  • EUR 495.00
  • EUR 300.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100150
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100151
  • EUR 439.00
  • EUR 133.00
  • EUR 1233.00
  • EUR 592.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cat Uromodulin Enzyme

20-abx073947
  • EUR 1609.00
  • EUR 328.00
  • EUR 230.00
  • 0.1 mg
  • 10 ug
  • 2 µg

CAT Rabbit pAb

A11777-100ul 100 ul
EUR 308

CAT Rabbit pAb

A11777-200ul 200 ul
EUR 459

CAT Rabbit pAb

A11777-20ul 20 ul
EUR 183

CAT Rabbit pAb

A11777-50ul 50 ul
EUR 223

CAT Rabbit pAb

A11780-100ul 100 ul
EUR 308

CAT Rabbit pAb

A11780-200ul 200 ul
EUR 459

CAT Rabbit pAb

A11780-20ul 20 ul
EUR 183

CAT Rabbit pAb

A11780-50ul 50 ul
EUR 223

Catalase (CAT) Antibody

20-abx132110
  • EUR 272.00
  • EUR 592.00
  • EUR 314.00
  • EUR 154.00
  • EUR 230.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx132122
  • EUR 300.00
  • EUR 718.00
  • EUR 384.00
  • EUR 154.00
  • EUR 244.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PKA CAT Antibody

abx147539-100ug 100 ug
EUR 439

Catalase (CAT) Antibody

20-abx148940
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx141181
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul

Catalase (CAT) Antibody

abx025488-100ul 100 ul
EUR 523

Catalase (CAT) Antibody

abx034033-400ul 400 ul
EUR 523

Catalase (CAT) Antibody

abx034033-80l 80 µl
EUR 286

CAT Rabbit pAb

A5275-100ul 100 ul
EUR 308

CAT Rabbit pAb

A5275-200ul 200 ul
EUR 459

CAT Rabbit pAb

A5275-20ul 20 ul Ask for price

CAT Rabbit pAb

A5275-50ul 50 ul Ask for price

Catalase (CAT) Antibody

abx430824-200ul 200 ul
EUR 384

Catalase (CAT) Antibody

20-abx242079
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx214177
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx175722
  • EUR 1302.00
  • EUR 620.00
  • 1 mg
  • 200 ug

Catalase (CAT) Antibody

20-abx171619
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Catalase (CAT) Antibody

abx231301-100ug 100 ug
EUR 481

Catalase (CAT) Antibody

20-abx326479
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

CAT Conjugated Antibody

C36312 100ul
EUR 397

Recombinant Human CAT

P0501 100ug
EUR 522.36
Description: Recombinant Human protein for CAT

Rat Catalase (CAT)

QY-E11654 96T
EUR 374

PKA CAT Antibody

AF5450 200ul
EUR 304
Description: PKA CAT Antibody detects endogenous levels of total PKA CAT.

PKA CAT Antibody

AF7746 200ul
EUR 376
Description: PKA CAT Antibody detects endogenous levels of PKA CAT.

PKA CAT Antibody

ABF5450 100 ug
EUR 438

Cat IgG (FITC)

65C-CH0202 1 mg
EUR 227
Description: Purified Cat IgG FITC conjugate

Cat IgG (HRP)

65C-CH0203 1 mg
EUR 248
Description: Purified Cat IgG HRP conjugate

b-cat antibody

70R-21460 50 ul
EUR 435
Description: Rabbit polyclonal b-cat antibody

Cat RBC antibody

20R-RR015 20 mg
EUR 327
Description: Rabbit polyclonal Cat RBC antibody

Cat RBC antibody

20R-RR016 5 mg
EUR 554
Description: Rabbit polyclonal Cat RBC antibody

Feline Cat IgG

31-1005 5 mg
EUR 175
Description: Feline (Cat) IgG Purified-Protein A

Normal Cat Serum

88R-1026 10 ml
EUR 250
Description: Normal Cat Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

pCMV-SPORT6-CAT

PVT14338 2 ug
EUR 495

pCEP4- CAT Plasmid

PVT1038 2 ug
EUR 266

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) Normal Uterus Whole tissue lysate

MRL-1332 1 mg
EUR 773

Monkey (Cyno.) Normal Uterus Whole tissue lysate

MCL-1222 1 mg
EUR 773

Human Adult Uterus (Normal) Whole tissue lysate

HAL-1317 1 mg
EUR 524

FFPE Genomic DNA - Human Tumor Tissue: Uterus

D2235274 2 ug
EUR 821
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Human Tumor Tissue: Uterus

P1235274 1 mg
EUR 320
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Tissue: Uterus

P3235274 0.1 mg
EUR 408
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Uterus

T1234274 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Uterus

T2234274 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor

T2235275 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Phospho-CAT (Y386) Antibody

1-CSB-PA070042
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Phospho-CAT (Y386). Recognizes Phospho-CAT (Y386) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

PKA? cat Polyclonal Antibody

ES6743-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against PKA? cat from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

PKA? cat Polyclonal Antibody

ES6743-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against PKA? cat from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

PKA? cat Polyclonal Antibody

ES6744-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against PKA? cat from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

PKA? cat Polyclonal Antibody

ES6744-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against PKA? cat from Human. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

CAT-4 Polyclonal Antibody

ES7231-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against CAT-4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

CAT-4 Polyclonal Antibody

ES7231-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against CAT-4 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

Sheep CAT ELISA Kit

ESC0093 96Tests
EUR 521

Mouse CAT ELISA Kit

EMC0093 96Tests
EUR 521

CPN cat Polyclonal Antibody

ES2041-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against CPN cat from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

CPN cat Polyclonal Antibody

ES2041-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against CPN cat from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

PKA?/? cat Polyclonal Antibody

ES3220-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against PKA?/? cat from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, IF, WB, ELISA

Growth of a New Internally Managed One-Step Actual-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar 

Enterovirus A71 (EV-A71) is a number one explanation for hand-foot-and-mouth illness (HFMD) and could be related to extreme neurological problems. EV-A71 strains could be labeled into seven genogroups, A-H, on the premise of the VP1 capsid protein gene sequence. Genogroup A contains the prototype pressure; genogroups B and C are accountable of main outbreaks worldwide, however little is thought in regards to the others, significantly genogroups E and F, which have been lately recognized in Africa and Madagascar, respectively.

The circulation of EV-A71 within the African area is poorly recognized and possibly underestimated. A fast and particular assay for detecting all genogroups of EV-A71 is required. On this examine, we developed a real-time RT-PCR assay with a aggressive inside management (IC). The primers and TaqMan probe particularly goal the genomic area encoding the VP1 capsid protein. Various EV-A71 RNAs had been efficiently amplified from the genogroups A, B, C, D, E, and F, with related sensitivity and strong reproducibility. Neither cross response with different EVs nor main interference with the aggressive IC was detected.

Experimentally spiked stool and plasma specimens supplied constant and reproducible outcomes, and validated the usefulness of the IC for demonstrating the presence of PCR inhibitors in samples. The evaluation in an African laboratories community of 1889 untyped enterovirus isolates detected 15 EV-A71 of various genogroups. This particular real-time RT-PCR assay offers a sturdy and delicate technique for the detection of EV-A71 in organic specimens and for the epidemiological monitoring of EV-A71 together with its lately found genogroups.

 

Exploration of turn-positive RT-PCR outcomes and components associated to therapy end result in COVID-19: A retrospective cohort examine

The reason for some sufferers with detrimental RT-PCR outcomes skilled turn-positive after therapy stays unclear. As well as, understanding the correlation between adjustments in medical information in the midst of COVID-19 and therapy outcomes is of nice significance in figuring out the prognosis of COVID-19. To carry out trigger evaluation of RT-PCR turn-positive and the efficient screening components associated to therapy end result in COVID-19.

Medical information, together with medical manifestations, laboratory checks, radiography outcomes, therapy strategies and outcomes, had been retrospectively collected and analyzed from January to March 2020 in Renmin Hospitals of Wuhan College. 116 COVID-19 sufferers (40 in recurrent group, 29 in recovered group and 47 in unrecovered group) had been recruited. Within the recurrent group, white blood cell, Neutrophils, prothrombin time, activated partial thromboplastin time, CD3, CD4, CD8, ratio of CD4/CD8, IgG and C4 complement had been of serious distinction among the many baseline, detrimental and turn-positive time factors. CD19 and CT scan outcomes had been discovered notable distinction between recurrent group and recovered group. Odds from CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT scan outcomes validated associations with medical outcomes of COVID-19.

The so-called recurrence in some COVID-19 sufferers could also be because of the false-negative of nucleic acid check outcomes from nasopharyngeal swabs. Ranges of CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT outcomes had been considerably correlated with the result of COVID-19. The mobile immunity check might be helpful to additional display the reliability of RT-PCR check on the premise of CT photographs.

 

 

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