A Comparative Research of Some Procedures for Isolation of Fruit DNA of Enough High quality

A Comparative Analysis of Some Procedures for Isolation of Fruit DNA of Sufficient Top quality for PCR-Based Assays

  • Meals fraud has been and nonetheless is a matter throughout the meals commerce. It is detectable by plenty of approaches, harking back to extreme effectivity liquid chromatography (HPLC), chemometric assays, or DNA-based methods, each with its private drawbacks. This work addresses one major drawback of DNA-based methods, significantly their sensitivity to inhibitors contained significantly matrices from which DNA is isolated.
  • We examined 5 enterprise kits and one in-house approach characterised by alternative routes of sample homogenization and DNA seize and purification.
  • Using these methods, DNA was isolated from 10 utterly completely different fruit species typically utilized in plant-based foodstuffs. The usual of the DNA was evaluated by UV-VIS spectrophotometry. Two sorts of qPCR assays have been used for DNA top quality testing: (i) Approach explicit for plant ITS2 space, (ii) methods explicit for explicit individual fruit species.
  • Based totally on the outcomes of real-time PCR assays, we now have been able to find two column-based kits and one magnetic carrier-based gear, which always equipped fruit DNA isolates of sufficient top quality for PCR-based assays useful for routine analysis and identification of explicit individual fruit species in meals merchandise.

Triplex real-time PCR assay for the authentication of camel-derived dairy and meat merchandise 

Authentication of dairy and meat merchandise is necessary to guarantee truthful competitors, client profit, and meals security. The big distinction in value between camel and cow milk could also be an incentive to adulterate camel dairy merchandise with cow-derived foodstuffs. Nevertheless, no research thus far have used triplex real-time PCR with an endogenous management to establish camel and cow origins in dairy and meat merchandise.

On this examine, we developed a triplex real-time PCR assay primarily based on amplification of mitochondrial 12S ribosomal DNA for the authentication of camel-derived dairy and meat merchandise. This technique was utilized to establish camel and cow DNA in milk, yogurt, cheese, milk powder, milk beverage, meat merchandise, and mixtures with milk and meat. Concentrations as little as 1 to five% and 0.1% camel milk and meat, respectively, had been detected within the mixtures, and 1 to five% and 0.1% cow milk and meat, respectively, had been recognized by way of this strategy.

The boundaries of detection had been 0.005 to 0.0025 ng, 0.05 to 0.001 ng, 0.001 to 0.0005 ng, and 0.00025 to 0.0001 ng of DNA in camel milk, camel yogurt, business camel milk beverage, and camel meat, and from 0.0025 to 0.001 ng, 0.5 to 0.001 ng, 1 to 0.05 ng, 0.01 ng, 0.001 ng, 0.0005 to 0.00025 ng, 0.0005 to 0.00025 ng, and 0.005 ng of DNA from cow milk, yogurt, cheese, acidic whey, milk powder, beef, beef jerky, and beef sausage, respectively. Totally different dairy and meat samples of camel and cow origins had a spread of authentication limits and limits of detection. The designed triplex real-time PCR assay was proven to be a selected, delicate, and environment friendly approach for the identification of camel and cow DNA in foodstuffs.

q-es
q-es

cDNA from Human Adult Normal Tissue: Uterus: Cervix

C1234275 40 reactions
EUR 451.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus: Corpus

C1234276-10 10 reactions
EUR 277.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Uterus: Fundus

C1234278-10 10 reactions
EUR 277.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Cervix

C1534275 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix

C1534275-Cy 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-48T 48T
EUR 529.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

DLR-CAT-Hu-96T 96T
EUR 684
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit

DLR-CAT-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-48T 48T
EUR 658.8
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit

DLR-CAT-Ra-96T 96T
EUR 861.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-48Tests 48 Tests
EUR 523.2

Human Catalase (CAT) ELISA Kit

RD-CAT-Hu-96Tests 96 Tests
EUR 721.2

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-48Tests 48 Tests
EUR 639.6

Mouse Catalase (CAT) ELISA Kit

RD-CAT-Mu-96Tests 96 Tests
EUR 888

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-48Tests 48 Tests
EUR 668.4

Rat Catalase (CAT) ELISA Kit

RD-CAT-Ra-96Tests 96 Tests
EUR 930

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-48Tests 48 Tests
EUR 546

Human Catalase (CAT) ELISA Kit

RDR-CAT-Hu-96Tests 96 Tests
EUR 754.8

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-48Tests 48 Tests
EUR 668.4

Mouse Catalase (CAT) ELISA Kit

RDR-CAT-Mu-96Tests 96 Tests
EUR 928.8

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-48Tests 48 Tests
EUR 699.6

Rat Catalase (CAT) ELISA Kit

RDR-CAT-Ra-96Tests 96 Tests
EUR 973.2

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine UTERUS 5 EA*

57153-2 5 EA
EUR 245.04

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 216.6

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 241.8

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 216.6

Uterus Tissue Slide (Adenocarcinoma)

11-404-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenocarcinoma)

11-404-4um 4 um
EUR 216.6

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 241.8

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 216.6

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 216.6

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 927.6

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 752.1
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Corpus Membrane Lysate

XBL-11037 0.1 mg
EUR 619.8
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate

XBL-11040 0.1 mg
EUR 619.8
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 213.6
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 232.8
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

CAT/ Rat CAT ELISA Kit

ELA-E0242r 96 Tests
EUR 1063.2

Rabbit UTERUS Y 25 EA*

41253-2 25 EA
EUR 382.07

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-10um 10 um
EUR 241.8

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-4um 4 um
EUR 216.6

Total RNA from Lupus: Uterus

R1236274Lup-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 424.8

Novo? cDNA Kit

M1165-25
EUR 320.4

Evo? cDNA Supermix

M1168-100
EUR 457.2

Evo? cDNA Supermix

M1168-25
EUR 320.4

Novo? cDNA Supermix

M1169-100
EUR 529.2

Novo? cDNA Supermix

M1169-25
EUR 346.8

CAT antibody

70R-16187 50 ul
EUR 522
Description: Rabbit polyclonal CAT antibody

Cat IgM

31C-CH0213 1 mg
EUR 470.4
Description: Purified Cat IgM

CAT antibody

20C-CR1111RP 500 ul
EUR 314.4
Description: Rabbit polyclonal CAT antibody

CAT Antibody

36312-100ul 100ul
EUR 302.4

CAT antibody

10R-3584 100 ul
EUR 871.2
Description: Mouse monoclonal CAT antibody

CAT antibody

10R-3585 100 ul
EUR 829.2
Description: Mouse monoclonal CAT antibody

CAT siRNA

20-abx900807
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx910366
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CAT siRNA

20-abx910367
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

CAT Antibody

1-CSB-PA599838
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200

CAT Antibody

1-CSB-PA070043
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000

CAT Antibody

1-CSB-PA942303
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

CAT Antibody

1-CSB-PA004563GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

CAT Antibody

1-CSB-PA004980
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

pOTB7-CAT

PVT18217 2 ug
EUR 277.2

pCas9- CAT

PVT11001 2 ug
EUR 361.2

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 393.6
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-10um 10 um
EUR 241.8

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-4um 4 um
EUR 216.6

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-10um 10 um
EUR 241.8

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-4um 4 um
EUR 216.6

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 405.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Catalase (10,000-15,000 U/mg), Bovine Liver

CAT-01 25 mU
EUR 634.8

Catalase (2000-4000 U/mg), Aspergillus Niger

CAT-02 25 mU
EUR 781.2

Catalase (4000-6000 U/mg), Bovine Liver

CAT-03 5 mU
EUR 416.4

Catalase (>40,000 u/mg), Bovine Liver

CAT-04 1 mg
EUR 343.2

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 5mL
EUR 127.2

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 534

Evo™ cDNA Kit

M1164-100 Ask for price

Evo™ cDNA Kit

M1164-25 Ask for price

Novo? Transcriptome cDNA Kit

M1167-100
EUR 1142.4

Novo? Transcriptome cDNA Kit

M1167-25
EUR 529.2

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 370.8

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 498

FFPE Genomic DNA - Human Tumor Tissue: Uterus

D2235274 2 ug
EUR 985.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Cyno.) Normal Uterus Whole tissue lysate

MCL-1222 1 mg
EUR 927.6

Monkey (Rhesus) Normal Uterus Whole tissue lysate

MRL-1332 1 mg
EUR 927.6

Total Protein from Human Tumor Tissue: Uterus

P1235274 1 mg
EUR 384
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Human Adult Uterus (Normal) Whole tissue lysate

HAL-1317 1 mg
EUR 628.8

Membrane Protein from Human Tumor Tissue: Uterus

P3235274 0.1 mg
EUR 489.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Paraffin Tissue Section - Human Adult Normal: Uterus

T2234274 5 slides
EUR 253.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor

T2235275 5 slides
EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Adult Normal: Uterus

T1234274 5 slides
EUR 418.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total RNA from Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Cat IgG (FITC)

65C-CH0202 1 mg
EUR 272.4
Description: Purified Cat IgG FITC conjugate

Cat IgG (HRP)

65C-CH0203 1 mg
EUR 297.6
Description: Purified Cat IgG HRP conjugate

CAT Rabbit pAb

A11777-100ul 100 ul
EUR 369.6

CAT Rabbit pAb

A11777-200ul 200 ul
EUR 550.8

CAT Rabbit pAb

A11777-20ul 20 ul
EUR 219.6

CAT Rabbit pAb

A11777-50ul 50 ul
EUR 267.6

CAT Rabbit pAb

A11780-100ul 100 ul
EUR 369.6

CAT Rabbit pAb

A11780-200ul 200 ul
EUR 550.8

CAT Rabbit pAb

A11780-20ul 20 ul
EUR 219.6

CAT Rabbit pAb

A11780-50ul 50 ul
EUR 267.6

b-cat antibody

70R-21460 50 ul
EUR 522
Description: Rabbit polyclonal b-cat antibody

Cat RBC antibody

20R-RR015 20 mg
EUR 392.4
Description: Rabbit polyclonal Cat RBC antibody

Cat RBC antibody

20R-RR016 5 mg
EUR 664.8
Description: Rabbit polyclonal Cat RBC antibody

Feline Cat IgG

31-1005 5 mg
EUR 210
Description: Feline (Cat) IgG Purified-Protein A

Catalase (CAT) Antibody

20-abx148940
  • EUR 510.00
  • EUR 410.40
  • 100 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx171619
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

Catalase (CAT) Antibody

20-abx141181
  • EUR 427.20
  • EUR 644.40
  • EUR 260.40
  • 100 ul
  • 200 ul
  • 30 ul

Catalase (CAT) Antibody

20-abx132110
  • EUR 326.40
  • EUR 710.40
  • EUR 376.80
  • EUR 184.80
  • EUR 276.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx132122
  • EUR 360.00
  • EUR 861.60
  • EUR 460.80
  • EUR 184.80
  • EUR 292.80
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

PKA CAT Antibody

abx147539-100ug 100 ug
EUR 526.8

CAT Rabbit pAb

A5275-100ul 100 ul
EUR 369.6

CAT Rabbit pAb

A5275-200ul 200 ul
EUR 550.8

CAT Rabbit pAb

A5275-20ul 20 ul Ask for price

CAT Rabbit pAb

A5275-50ul 50 ul Ask for price

Catalase (CAT) Antibody

20-abx100146
  • EUR 427.20
  • EUR 159.60
  • EUR 1195.20
  • EUR 594.00
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100147
  • EUR 427.20
  • EUR 159.60
  • EUR 1195.20
  • EUR 594.00
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100148
  • EUR 427.20
  • EUR 159.60
  • EUR 1195.20
  • EUR 594.00
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100149
  • EUR 427.20
  • EUR 159.60
  • EUR 1195.20
  • EUR 594.00
  • EUR 360.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100150
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx100151
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Cat Uromodulin Enzyme

20-abx073947
  • EUR 1930.80
  • EUR 393.60
  • EUR 276.00
  • 0.1 mg
  • 10 ug
  • 2 µg

Catalase (CAT) Antibody

20-abx004037
  • EUR 493.20
  • EUR 710.40
  • 100 ul
  • 200 ul

Catalase (CAT) Antibody

20-abx125619
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx125620
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx111432
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Catalase (CAT) Antibody

abx034033-400ul 400 ul
EUR 627.6

Catalase (CAT) Antibody

abx034033-80l 80 µl
EUR 343.2

Catalase (CAT) Antibody

abx025488-100ul 100 ul
EUR 627.6

PKA CAT Antibody

ABF5450 100 ug
EUR 525.6

Catalase (CAT) Antibody

abx231301-100ug 100 ug
EUR 577.2

Catalase (CAT) Antibody

20-abx242079
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Catalase (CAT) Antibody

20-abx323535
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Catalase (CAT) Antibody

20-abx326479
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Catalase (CAT) Antibody

abx430824-200ul 200 ul
EUR 460.8

Growth of a New Internally Managed One-Step Actual-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar 

Enterovirus A71 (EV-A71) is a number one explanation for hand-foot-and-mouth illness (HFMD) and could be related to extreme neurological problems. EV-A71 strains could be labeled into seven genogroups, A-H, on the premise of the VP1 capsid protein gene sequence. Genogroup A contains the prototype pressure; genogroups B and C are accountable of main outbreaks worldwide, however little is thought in regards to the others, significantly genogroups E and F, which have been lately recognized in Africa and Madagascar, respectively.

The circulation of EV-A71 within the African area is poorly recognized and possibly underestimated. A fast and particular assay for detecting all genogroups of EV-A71 is required. On this examine, we developed a real-time RT-PCR assay with a aggressive inside management (IC). The primers and TaqMan probe particularly goal the genomic area encoding the VP1 capsid protein. Various EV-A71 RNAs had been efficiently amplified from the genogroups A, B, C, D, E, and F, with related sensitivity and strong reproducibility. Neither cross response with different EVs nor main interference with the aggressive IC was detected.

Experimentally spiked stool and plasma specimens supplied constant and reproducible outcomes, and validated the usefulness of the IC for demonstrating the presence of PCR inhibitors in samples. The evaluation in an African laboratories community of 1889 untyped enterovirus isolates detected 15 EV-A71 of various genogroups. This particular real-time RT-PCR assay offers a sturdy and delicate technique for the detection of EV-A71 in organic specimens and for the epidemiological monitoring of EV-A71 together with its lately found genogroups.

 

Exploration of turn-positive RT-PCR outcomes and components associated to therapy end result in COVID-19: A retrospective cohort examine

The reason for some sufferers with detrimental RT-PCR outcomes skilled turn-positive after therapy stays unclear. As well as, understanding the correlation between adjustments in medical information in the midst of COVID-19 and therapy outcomes is of nice significance in figuring out the prognosis of COVID-19. To carry out trigger evaluation of RT-PCR turn-positive and the efficient screening components associated to therapy end result in COVID-19.

Medical information, together with medical manifestations, laboratory checks, radiography outcomes, therapy strategies and outcomes, had been retrospectively collected and analyzed from January to March 2020 in Renmin Hospitals of Wuhan College. 116 COVID-19 sufferers (40 in recurrent group, 29 in recovered group and 47 in unrecovered group) had been recruited. Within the recurrent group, white blood cell, Neutrophils, prothrombin time, activated partial thromboplastin time, CD3, CD4, CD8, ratio of CD4/CD8, IgG and C4 complement had been of serious distinction among the many baseline, detrimental and turn-positive time factors. CD19 and CT scan outcomes had been discovered notable distinction between recurrent group and recovered group. Odds from CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT scan outcomes validated associations with medical outcomes of COVID-19.

The so-called recurrence in some COVID-19 sufferers could also be because of the false-negative of nucleic acid check outcomes from nasopharyngeal swabs. Ranges of CD3, CD4, CD8, CD19, IgM, C3 complement, C4 complement and CT outcomes had been considerably correlated with the result of COVID-19. The mobile immunity check might be helpful to additional display the reliability of RT-PCR check on the premise of CT photographs.

 

 

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