New multiplex conventional PCR and quadruplex real-time PCR assays for one-tube

Seven Years Leptospirosis Observe-Up in a Essential Care Unit of a French Metropolitan Hospital; Position of Actual Time PCR for a Fast and Acute Prognosis

(1) Background: Leptospirosis an an infection can lead to plenty of organ failure, requiring hospitalization in an intensive care unit for supportive care, along with initiation of an tailor-made antibiotic treatment. Attaining a quick prognosis is decisive throughout the administration of these victims.

(2) Methods: We present proper right here a consider of leptospirosis circumstances acknowledged throughout the intensive care unit of our hospital over seven years. Medical and natural data have been gathered, and we in distinction the variations in relation to diagnostic approach.

 (3) Outcomes: Molecular biology approach by Polymerase Chain Response (PCR) allowed quick and reliable prognosis when carried out throughout the first days after the indicators began. Moreover, we acknowledged that sampling blood and urine for PCR was further setting pleasant than performing PCR on only one kind of natural sample.

 (4) Conclusions: Our outcomes affirm the effectivity of PCR for the quick prognosis of leptospirosis and suggest that testing every blood and urine early throughout the sickness could improve prognosis.

New multiplex standard PCR and quadruplex real-time PCR assays for one-tube detection of Phyllosticta citricarpa, Elsinoë fawcettii, Elsinoë australis, and Pseudocercospora angolensis in Citrus: improvement and validation

 

Phyllosticta citricarpa, Elsinoë fawcettii, Elsinoë australis, and Pseudocercospora angolensis are main pathogens of citrus crops worldwide and might trigger non-characteristic signs which will result in confusion relating to the causative agent. These fungi are topic to worldwide phytosanitary rules, and testing on fruits or leaves requires correct and easy-to-use instruments.

New multiplex standard PCR and real-time PCR assays have been developed right here to realize extremely correct simultaneous detection of all 4 fungal pathogens in fruit tissues. We designed new oligonucleotide mixtures for P. citricarpa, E. fawcettii, and E. australis and mixed them with already out there primers and hydrolysis probes for use in both PCR assay. The restrict of detection for multiplex standard PCR was as little as 100 pg μL-1 for P. citricarpa, E. fawcettii, and E. australis and 10 pg μL-1 of goal DNA per response tube for P. angolensis.

The quadruplex real-time PCR assay efficiently yielded repeatable constructive outcomes with as little as 242, 243, 241, and 242 plasmidic copies of goal DNA of P. citricarpa, E. fawcettii, E. australis, and P. angolensis, respectively. Furthermore, evaluation of 60 naturally contaminated citrus samples yielded 100% concordant outcomes by each assays. Our validation experiment revealed that the multiplex real-time PCR assay confirmed excessive specificity besides a cross-reaction with P. paracitricarpa DNA.

Sensitivity, repeatability, reproducibility, and robustness have been verified, and the assay might be used following totally different DNA extraction procedures, supporting health for routine evaluation. These new multiplex instruments must be of nice curiosity as cost-effective options for regulatory authorities and diagnostic laboratories, enabling testing for 4 essential pathogens in single-tube reactions. KEY POINTS: • Improvement of recent standard PCR and qPCR assays for 4 citrus pathogens. • Very low limits of detection have been discovered for multiplex standard PCR. • qPCR had excessive specificity, sensitivity, repeatability, reproducibility, and robustness.

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Human Gland Salivary Tissue Lysate

IHUGLSTL100UG each
EUR 1067
Description: Human Gland Salivary Tissue Lysate

Human Gland Salivary Lysate Membrane Fraction

IHUGLSTLM100UG each
EUR 1413
Description: Human Gland Salivary Lysate Membrane Fraction

Total RNA - Human Adult Normal Tissue: Salivary Gland

R1234212-10 10 ug
EUR 223

Total Protein - Human Adult Normal Tissue: Salivary Gland

P1234212 1 mg
EUR 351

Membrane Protein - Human Adult Normal Tissue: Salivary Gland

P3234212 0.1 mg
EUR 535

Human Salivary Gland Tissue Slide (Abnormal) (5 slides/pk)

HTS-12128 1 pk
EUR 343.2

Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7)

1-CSB-YP321587AXQ
  • EUR 814.80
  • EUR 402.00
  • EUR 2606.40
  • EUR 1261.20
  • EUR 1730.40
  • EUR 522.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in Yeast

Aedes aegypti 37KDA salivary gland allergen Aed a 2 (D7)

1-CSB-EP321587AXQ
  • EUR 733.20
  • EUR 370.80
  • EUR 2192.40
  • EUR 1126.80
  • EUR 1461.60
  • EUR 476.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Aedes aegypti 37KDA salivary gland allergen Aed a 2(D7) expressed in E.coli

Parotid gland disease spectrum (parotidean cancer progression) tissue array

OR801 each
EUR 306
Description: Parotid gland disease spectrum (parotidean cancer progression) tissue array, 80 cases/80 cores

Human Adult Salivary (Normal) Gland Whole tissue lysate

HAL-20103 1 mg
EUR 927.6

Normal Rhesus Salivary gland (single section per slide) (5 slides/pack)

RsFPT116 5 slides
EUR 150

cDNA - Monkey (Rhesus) Normal Tissue: Parotid

C1534190 40 reactions
EUR 578

Human Salivary Gland Tissue Slide (Mucoepidermoid carcinoma) (5 slides/pk)

HTS-12109 1 pk
EUR 343.2

cDNA - Monkey (Cynomolgus) Normal Tissue: Parotid

C1534190-Cy 40 reactions
EUR 578

Parotid Dissociation System 2 (Parotid), Mouse and Rat

4-20392 ea Ask for price

Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat

4-20394 ea Ask for price

Human Salivary IGF(salivary IGF) ELISA Kit

QY-E05383 96T
EUR 433.2

Rat Salivary Amylase ELISA kit

E02S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Salivary Amylase ELISA kit

E02S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Salivary Amylase ELISA kit

E02S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat

4-20393 ea Ask for price

Rat Salivary duct autoantibody ELISA kit

E02S0244-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Salivary duct autoantibody ELISA kit

E02S0244-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Salivary duct autoantibody ELISA kit

E02S0244-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Salivary duct autoantibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Amylase Alpha 1, Salivary ELISA kit

E02A0476-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Amylase Alpha 1, Salivary in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Amylase Alpha 1, Salivary ELISA kit

E02A0476-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Amylase Alpha 1, Salivary in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Amylase Alpha 1, Salivary ELISA kit

E02A0476-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Amylase Alpha 1, Salivary in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Amylase Alpha 1 Salivary ELISA Kit

IRTAMY1KT each
EUR 754
Description: Rat Amylase Alpha 1 Salivary ELISA Kit

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKN43435-48T 48T
EUR 390.46

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKN43435-5x96T 5x96T
EUR 2649.55

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKN43435-96T 96T
EUR 557.8

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKU02324-48T 48T
EUR 573.02

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKU02324-5x96T 5x96T
EUR 3888.35

Rat Salivary Alpha Amylase (AMY1A) ELISA Kit

EKU02324-96T 96T
EUR 818.6

anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS

ELA-E0500r 96 Tests
EUR 1063.2

Rat Amylase Alpha 1, Salivary (AMY1) Protein

20-abx652497
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat anti parotid duct antibody ELISA kit

E02A2104-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Amylase Alpha 1, Salivary (AMY1) CLIA Kit

20-abx492794
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

abx256705-96tests 96 tests
EUR 848.4

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

20-abx155191
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

DLR-AMY1-Ra-48T 48T
EUR 633.6
Description: A sandwich quantitative ELISA assay kit for detection of Rat Amylase Alpha 1, Salivary (AMY1) in samples from serum, plasma or other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

DLR-AMY1-Ra-96T 96T
EUR 828
Description: A sandwich quantitative ELISA assay kit for detection of Rat Amylase Alpha 1, Salivary (AMY1) in samples from serum, plasma or other biological fluids.

Rat AMY1(Amylase Alpha 1, Salivary) ELISA Kit

ER0731 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.938 ng/ml

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

SEB482Ra-10x96wellstestplate 10x96-wells test plate
EUR 5850.59
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Amylase Alpha 1, Salivary (AMY1) in serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

SEB482Ra-1x48wellstestplate 1x48-wells test plate
EUR 586.99
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Amylase Alpha 1, Salivary (AMY1) in serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

SEB482Ra-1x96wellstestplate 1x96-wells test plate
EUR 787.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Amylase Alpha 1, Salivary (AMY1) in serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

SEB482Ra-5x96wellstestplate 5x96-wells test plate
EUR 3182.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Amylase Alpha 1, Salivary (AMY1) in serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

4-SEB482Ra
  • EUR 5911.20
  • EUR 3122.40
  • EUR 787.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Amylase Alpha 1, Salivary (AMY1) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RD-AMY1-Ra-48Tests 48 Tests
EUR 640.8

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RD-AMY1-Ra-96Tests 96 Tests
EUR 890.4

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RDR-AMY1-Ra-48Tests 48 Tests
EUR 669.6

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RDR-AMY1-Ra-96Tests 96 Tests
EUR 931.2

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RDR-AMY1-Ra-48T 48T
EUR 488.78
Description: serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RDR-AMY1-Ra-96T 96T
EUR 698.25
Description: serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

DL-AMY1-Ra 96T
EUR 454
Description: serum, plasma or other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

DLR-AMY1-Ra 96T
EUR 477
Description: serum, plasma or other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RD-AMY1-Ra-48T 48T
EUR 465.5
Description: serum, plasma and other biological fluids.

Rat Amylase Alpha 1, Salivary (AMY1) ELISA Kit

RD-AMY1-Ra-96T 96T
EUR 665
Description: serum, plasma and other biological fluids.

Rat AMY1 (Amylase Alpha 1, Salivary) ELISA Kit

EKE62426-5x96T 5x96T
EUR 3124.08

Rat AMY1 (Amylase Alpha 1, Salivary) ELISA Kit

EKE62426-96T 96T
EUR 657.7

Parotid Membrane Tumor Lysate

XBL-10807 0.1 mg
EUR 1023
Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Salivary Sample Collection Kit

55R-IB76305 500 tests
EUR 361.2
Description: Salivary sample collection kit for the detection of free Salivary proteins and enzymes in the research laboratory

Pig Salivary Amylase ELISA kit

E07S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Salivary Amylase ELISA kit

E07S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Salivary Amylase ELISA kit

E07S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Salivary Amylase ELISA kit

E08S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Salivary Amylase ELISA kit

E08S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Salivary Amylase ELISA kit

E08S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Salivary Free Estriol ELISA Kit

DEIA-LL101 96T
EUR 850.8
Description: Competitive immunoenzymatic colorimetric method for quantitative measurement of Estriol concentration in saliva.

Goat Salivary Amylase ELISA kit

E06S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Salivary Amylase ELISA kit

E06S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Salivary Amylase ELISA kit

E06S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pancreas Dissociation System 6 (Acinar, rat parotid), Rat

4-20376 ea Ask for price

ELISA kit for Rat AMY1 (Amylase Alpha 1, Salivary)

E-EL-R2544 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Rat AMY1 (Amylase Alpha 1, Salivary) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Rat AMY1 (Amylase Alpha 1, Salivary)

ELK7087 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Amylase Alpha 1, Salivary from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Parotid secretory protein Antibody

abx431530-200ul 200 ul
EUR 460.8

Parotid secretory protein Antibody

abx431531-200ul 200 ul
EUR 343.2

Mouse Salivary Amylase ELISA kit

E03S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Salivary Amylase ELISA kit

E03S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Salivary Amylase ELISA kit

E03S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Salivary Amylase ELISA kit

E01S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Salivary Amylase ELISA kit

E01S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Salivary Amylase ELISA kit

E01S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit SALIVARY SUBMAX Y 50 EA*

41243-2 50 EA
EUR 382.07

Rabbit Salivary Amylase ELISA kit

E04S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Salivary Amylase ELISA kit

E04S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Salivary Amylase ELISA kit

E04S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Salivary Amylase ELISA kit

E09S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Salivary Amylase ELISA kit

E09S0191-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Salivary Amylase ELISA kit

E09S0191-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bovine SALIVARY SUBMAXILLARY 50 EA*

57143-2 50 EA
EUR 555.64

AMY1 ELISA Kit| Rat Amylase Alpha 1, Salivary ELISA Kit

EF017538 96 Tests
EUR 826.8

Guinea pig Salivary Amylase ELISA kit

E05S0191-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Salivary Amylase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

SEM and qRT-PCR revealed quercetin inhibits morphogenesis of Aspergillus flavus conidia by way of modulating calcineurin-Crz1 signalling pathway

Aspergillus flavus: exploits various mechanisms to outlive throughout publicity to antifungal brokers together with morphogenesis. Germination of dormant conidia entails cascades of reactions built-in into the signalling pathway. This research paperwork the impact of phytochemical-quercetin on A. flavus throughout germination of conidia utilizing scanning electron microscopy (SEM).

 

Vital inhibition of conidial swelling of A. flavus compared to management was noticed at Four and seven h Quantitative real-time PCR for genes from calcium signalling pathway and heat-shock proteins household confirmed up-regulation of warmth shock (Hsp70 and Hsp90) and calcium signalling pathway genes (calcium-transporting ATPase and calmodulin) in response to quercetin at preliminary Four h compared to management pattern whereas up-regulation of Hsp70, calcineurin and transcription issue Crz1, have been noticed in each the handled samples. Gene encoding for calcium-kinase, cAMP, Rho-gdp, Plc and Pkc confirmed a constitutively greater degree of expression in quercetin-treated pattern compared to management at each time factors. These knowledge confirmed a transparent response from genes encoding calcineurin-Crz1 signalling pathways and should discover its software within the screening of antifungal brokers.

 

Abbreviations: Hsp: Hear shock protein; MIC: Minimal Inhibitory Focus; SEM: Scanning Electron Microscopy; qRT-PCR: Quantitative Actual-Time Polymerase Chain Response.

Quantitative Actual-Time PCR Analysis of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

 

MicroRNAs (miRNAs) are single stranded, non-coding RNA molecules that usually regulate gene expression on the post-transcriptional degree by binding to partially complementary goal websites within the 3′ untranslated area (UTR) of messenger RNA (mRNA), which reduces the mRNA’s translation and stability.

The miRNA expression profiles in numerous organs and tissues of mice have been investigated, however commonplace strategies for the purification and quantification of miRNA in mouse kidney haven’t been out there. We have now established an efficient and dependable technique for extracting and evaluating miRNA expression in mouse kidney with renal interstitial fibrosis by quantitative reverse-transcription polymerase chain response (qRT-PCR).

The protocol required 5 steps: (1) creation of sham and unilateral ureteral obstruction (UUO) mice; (2) extraction of kidney samples from the UUO mice; (3) extraction of whole RNA, which incorporates miRNA, from the kidney samples; (4) complementary DNA (cDNA) synthesis with reverse transcription from miRNA; and (5) qRT-PCR utilizing the cDNA. Utilizing this protocol, we efficiently confirmed that in comparison with the controls, the expression of miRNA-3070-3p was considerably elevated and people of miRNA-7218-5p and miRNA-7219-5p have been considerably decreased within the kidneys of a mouse mannequin of renal interstitial fibrosis. This protocol can be utilized to find out the miRNA expression within the kidneys of mice with UUO.

 

Monkeypox Virus Real Time PCR Kit

ZD-0076-02 25 tests/kit Ask for price
Description: Monkeypox virus is the virus that causes the disease monkeypox in both humans and animals. Monkeypox virus is an Orthopoxvirus, a genus of the family Poxviridae that contains other viral species that target mammals. The virus is mainly found in tropical rainforest regions of central and West Africa. The primary route of infection is thought to be contact with the infected animals or their bodily fluids.The genome is not segmented and contains a single molecule of linear double-stranded DNA, 185000 nucleotides long.The Monkeypox Virus real time PCR Kit contains a specific ready-to-use system for the detection of the Monkeypox Virusthrough polymerase chain reaction (PCR) in the real-time PCR system. The master contains reagents and enzymes for the specific amplification of theMonkeypox VirusDNA. Fluorescence is emitted and measured by the real time systems ́ optical unit during the PCR. The detection of amplified Monkeypox Virus DNA fragment is performed in fluorimeter channelFAM with the fluorescent quencher BHQ1. DNA extraction buffer is available in the kit and serum or lesion exudate samples are used for the extraction of the DNA. In addition, the kit contains a system to identify possible PCR inhibition by measuring the HEX/VIC/JOE fluorescence of the internal control (IC). An external positive control defined as 1×107copies/ml is supplied which allow the determination of the gene load.

Monkeypox Virus Real Time PCR Kit

YJC70115NW-25T 25 tests/kit Ask for price
Description: The Bioperfectus Monkeypox Virus Real Time PCR Kit is an in vitro diagnostic test, based on real-time PCR technology, for the detection of DNA from the Monkeypox virus. Specimens can be obtained from human serum, lesion exudate samples and scab. BSL-2 facilities with standard BSL-2 work practices may be used for the test of t he Monkeypox virus.

Human Lung Cancer PCR Primer Library

HLUCPL-I 1 set
EUR 657.6

Human Schwann Cell PCR Primer Library

HSCH-I 1 set
EUR 657.6

0.2ML CLEAR STRIP CAPS FOR REAL TIME PCR

PCR-2CP-RT-C 125/pk
EUR 267.6
Description: PCR Plates & Tubes; PCR Strip Tubes and Strip Caps - Axygen

Novel Coronavirus COVID-19 (2019-nCoV) Real Time RT-PCR Kit

RR-0478-02 25 tests/kit
EUR 1189.2
Description: Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems.

AzuraQuant cDNA Synthesis Kit - 100 Reactions

AZ-1996 100 Reactions
EUR 446.4

AzuraQuant cDNA Synthesis Kit - 25 Reactions

AZ-1995 25 Reactions
EUR 183.6

Axygen Polyester ultra clear sealing film for real time PCR

AXY2152 PK100
EUR 382.8

Eztime Real - time PCRPremix 2x,Taqman

FYT105-100P 100 Preps 1.25 ml Ask for price

Eco 48 Real Time PCR system including software and loading dock

MOL6348 EACH
EUR 18466.8

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBR Green

FYT103-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT105-400P 400 Preps 1.25 ml x 4 Ask for price

Eztime Real - time PCRPremix 2x,Taqman, ROX

FYT106-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-100P 100 Preps 1.25 ml Ask for price

Eztime Real - time PCRPremix 2x,SYBRGreen , ROX

FYT104-400P 400 Preps 1.25 ml x 4 Ask for price

hsa-mir-521 Real-time RT-PCR Detection Kit

20-abx097600
  • EUR 661.20
  • EUR 944.40
  • EUR 477.60
  • 100 rxns
  • 200 rxns
  • 50 rxns

Green Dye One Step qRT-PCR Master Mix For Quantitative Real Time PCR With ROX Dye

MB1004-100Reactions 100 Reactions
EUR 392.4

Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

RR-0479-02 25 tests/kit
EUR 469.56
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

Azura PureView 1 Kb DNA Ladder - 100 Lanes

AZ-1101 100 Lanes
EUR 140.4

Azura PureView 1 Kb DNA Ladder - 500 Lanes

AZ-1105 500 Lanes
EUR 277.2

Azura PureView 1 Kb DNA Ladder - 1000 Lanes

AZ-1105-2 1000 Lanes
EUR 326.4

Azura PureView 250bp DNA Ladder - 100 Lanes

AZ-1121 100 Lanes
EUR 140.4

Azura PureView 250bp DNA Ladder - 500 Lanes

AZ-1125 500 Lanes
EUR 277.2

Azura PureView 250bp DNA Ladder - 1000 Lanes

AZ-1125-2 1000 Lanes
EUR 326.4

Azura PureView 100bp DNA Ladder - 100 Lanes

AZ-1131 100 Lanes
EUR 140.4

Azura PureView 100bp DNA Ladder - 500 Lanes

AZ-1135 500 Lanes
EUR 277.2

Azura PureView 100bp DNA Ladder - 1000 Lanes

AZ-1135-2 1000 Lanes
EUR 326.4

Azura PureView 50bp DNA Ladder - 100 Lanes

AZ-1151 100 Lanes
EUR 140.4

Azura PureView 50bp DNA Ladder - 500 Lanes

AZ-1155 500 Lanes
EUR 277.2

Azura PureView 50bp DNA Ladder - 1000 Lanes

AZ-1155-2 1000 Lanes
EUR 326.4

AzuraView GreenFast qPCR Blue Mix Lo Rox - 200 Rxn

AZ-2301 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Lo Rox - 500 Rxn

AZ-2305 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Lo Rox - 2000 Rxn

AZ-2320 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Lo Rox - 5000 Rxn

AZ-2325 5000 Rxn
EUR 2817.6

AzuraView GreenFast qPCR Blue Mix Hi Rox - 200 Rxn

AZ-2401 200 Rxn
EUR 214.8

AzuraView GreenFast qPCR Blue Mix Hi Rox - 500 Rxn

AZ-2405 500 Rxn
EUR 384

AzuraView GreenFast qPCR Blue Mix Hi Rox - 2000 Rxn

AZ-2420 2000 Rxn
EUR 1195.2

AzuraView GreenFast qPCR Blue Mix Hi Rox - 5000 Rxn

AZ-2421 5000 Rxn
EUR 2817.6

Mouse Gdnf Signaling Primer Library

MGDNF-I 1 set
EUR 657.6

Mouse Glucose Metabolism Primer Library

MGLM-I 1 set
EUR 657.6

Mouse Hippo Signaling Primer Library

MHPO-I 1 set
EUR 657.6

Mouse Hypoxia Signaling Primer Library

MHYP-I 1 set
EUR 657.6

Mouse Notch Signaling Primer Library

MNOT-I 1 set
EUR 657.6

Mouse Neurotransmitter Signaling Primer Library

MNTS-I 1 set
EUR 657.6

Mouse Integrin Signaling Primer Library

MINT-I 1 set
EUR 891.6

Mouse Oxidative Stress Primer Library

MOSL-I 1 set
EUR 657.6

Mouse Oncogene Primer Library

MONC-I 1 set
EUR 657.6

Mouse Apoptosis Primer Library

MPA-I 1 set
EUR 540

Mouse Ras Signaling Primer Library

MRAS-I 1 set
EUR 657.6

Mouse T-Cell Receptor Signaling Primer Library

MTCR-I 1 set
EUR 657.6

Mouse TGF Beta Signaling Primer Library

MTGFB-I 1 set
EUR 657.6

Mouse Tumor Invasion/Metastasis Primer Library

MTIM-I 1 set
EUR 540

Mouse Tumor Immunity Primer Library

MTIMU-I 1 set
EUR 657.6

Mouse p53 Signaling Primer Library

MTPS-I 1 set
EUR 540

Mouse WNT Signaling Primer Library

MWNT-I 1 set
EUR 657.6

Mouse Toll-like Receptor Signaling Primer Library

newitem 1 set
EUR 657.6

Mouse PI3K-AKT Signaling Primer Library

MAKT-I 1 set
EUR 657.6

Mouse Angiogenesis Primer Library

MAPL-I 1 set
EUR 540

Mouse Adherin - Tight Junction Primer Library

MATJ-I 1 set
EUR 657.6

Mouse Autophagy Primer Library

MATPL-I 1 set
EUR 540

Mouse Bdnf Signaling Primer Library

MBDNF-I 1 set
EUR 657.6

Mouse BMP Signaling Primer Library

MBMP-I 1 set
EUR 657.6

Mouse Cytokine Primer Library I

MCA-I 1 set
EUR 540

Mouse Cytokine Primer Library II

MCA-II 1 set
EUR 540

Mouse Cell Cycle Primer Library

MCC-I 1 set
EUR 540

Mouse Cytokine and Chemokine Receptor Primer Library

MCCR-I 1 set
EUR 774

Mouse CREB Signaling Primer Library

MCREB-I 1 set
EUR 657.6

Mouse DNA Damage Signaling Primer Library

MDDS-I 1 set
EUR 657.6

Mouse DNA Repair Primer Library I

MDRL-I 1 set
EUR 774

Mouse DNA Repair Primer Library II

MDRL-II 1 set
EUR 774

Mouse Adipogenesis Primer Library

MADG-I 1 set
EUR 657.6

Mouse EGFR Signaling Primer Library

MEGFR-I 1 set
EUR 657.6

Mouse Epithelial-Mesenchymal Transition Primer Library

MEMT-I 1 set
EUR 657.6

Mouse Estrogen Receptor Signaling Library

MESR-I 1 set
EUR 774

Mouse Fatty Liver Signaling Primer Library

MFTL-I 1 set
EUR 657.6

Mouse Jak/Stat Signaling Primer Library

MJAK-I 1 set
EUR 774

Mouse MAP Kinase Signaling Primer Library

MMAP-I 1 set
EUR 657.6

Mouse Myeloid Derived Suppressor Cell Primer Library

MMDSC-I 1 set
EUR 657.6

Mouse NFKappaB Primer Library

MNFKB-I 1 set
EUR 540

Human DNA Repair Primer Library I

HDRL-I 1 set
EUR 657.6

Human DNA Repair Primer Library II

HDRL-II 1 set
EUR 657.6

Human Drug Transporter I Primer Library

HDTP-I 1 set
EUR 657.6

Human Drug Detoxication I

HDTX-I 1 set
EUR 657.6

Human Drug Detoxication II

HDTX-II 1 set
EUR 774

Human EGFR Signaling Primer Library

HEGFR-I 1 set
EUR 657.6

Human Epithelial Mesenchymal Transition Primer Library

HEMT-I 1 set
EUR 657.6

Human Endothelial Cell Primer Library

HEND-I 1 set
EUR 657.6

Human ERBB Signaling Primer Library

HERBB-I 1 set
EUR 657.6

Human Estrogen Signaling Primer Library

HESR-I 1 set
EUR 774

Human Focal Adhesion Signaling Primer Library

HFCA-I 1 set
EUR 657.6

Human FGF Receptor Signaling Primer Library

HFGF-I 1 set
EUR 774

Human Fatty Acid and Lipid Metabolism Primer Library

HFLM-I 1 set
EUR 657.6

Human Gastric Cancer Primer Library

HGCPL-I 1 set
EUR 657.6

Human GDNF Signaling Primer Library

HGDNF-I 1 set
EUR 657.6

Human Glucose Metabolism Primer Library

HGLM-I 1 set
EUR 540

Human G-Protein Coupled Receptor Signaling Primer Library

HGPCR-I 1 set
EUR 657.6

Human Hedgehog Signaling Primer Library

HHOG-I 1 set
EUR 774

Human Homeobox Gene Primer Library

HHOX-I 1 set
EUR 774

Human Hippo Signaling Primer Pathway

HHPO-I 1 set
EUR 657.6

Human Heat Shock Protein Primer Library

HHSP-I 1 set
EUR 891.6

Human Hypoxia Signaling Primer Library

HHYP-I 1 set
EUR 657.6

Human Interferon Type I Signaling Primer Library

HIFN-I 1 set
EUR 657.6

Human Interferon Type II Signaling Primer Library

HIFN-II 1 set
EUR 657.6

Human IL17 Signaling Primer Library

HIL17-I 1 set
EUR 657.6

Human Insulin Signaling Primer Library

HINS-I 1 set
EUR 657.6

Human Integrin Signaling Primer Library

HINT-I 1 set
EUR 657.6

Human Actin Cytoskeleton Signaling Primer Library

HACS-I 1 set
EUR 657.6

Human Adipogenesis Primer Library

HADG-I 1 set
EUR 657.6

Human Adaptive & Innate Immune Response Primer Library

HAIIR-I 1 set
EUR 657.6

Human PI3K-AKT Signaling Primer Library

HAKT-I 1 set
EUR 540

Human Amino Acid Metabolism Primer Library

HAMM-I 1 set
EUR 774

Human Androgen Signaling Primer Library

HAND-I 1 set
EUR 657.6

Human Angiogenesis Primer Library

HAPL-I 1 set
EUR 540

Human Antigen Processing and Presentation Primer Library

HAPP-I 1 set
EUR 657.6

Human Atherosclerosis Primer Library

HATH-I 1 set
EUR 657.6

Human Adherin - Tight Junction Primer Library

HATJ-I 1 set
EUR 657.6

Human Autophagy Primer Library

HATPL-I 1 set
EUR 540

Human Alzheimer's Disease Primer Library

HAZD-I 1 set
EUR 657.6

Human B-Cell Receptor Signaling Primer Library

HBCR-I 1 set
EUR 657.6

Human BDNF Signaling Primer Library

HBDNF-I 1 set
EUR 657.6

Human Bladder Cancer Primer Library

HBLCP-I 1 set
EUR 657.6

Human BMP Signaling Primer Library

HBMP-I 1 set
EUR 657.6

Human Brain Cancer Primer Library

HBRC-I 1 set
EUR 657.6

Human Breast Cancer Signaling Primer Library

HBRCA-I 1 set
EUR 657.6

Human Cytokine Primer Library I

HCA-I 1 set
EUR 540

Human Cytokine Primer Library II

HCA-II 1 set
EUR 540

Human Cell Adhesion Primer Library

HCAD-I 1 set
EUR 774

Human cAMP Signaling Primer Library

HCAMP-I 1 set
EUR 657.6

Human Cancer Pathway Primer Library

HCAN-I 1 set
EUR 657.6

Human Calcium Regulation Pathway

HCAR-I 1 set
EUR 657.6

Human Cell Cycle Primer Library

HCC-I 1 set
EUR 540

Human Colon Cancer Primer Library I

HCCP-I 1 set
EUR 657.6

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